Sterile Technique for Bacterial Culturing Digital Badge

Contact Information

The Sterile Technique for Bacterial Culturing badge is a competency badge offered through the Department of Chemistry and Biochemistry. Completing this badge demonstrates the proper handling of sterile materials and bacterial cultures in a biochemical laboratory setting.

Earning Criteria

Participants earning this badge are required to demonstrate appropriate laboratory safety skills and the effective transfer of media, inoculate liquid media with a bacterial culture, and streak bacterial culture on solid plates to isolate individual bacterial colonies.

Competencies Demonstrated

1. Use aseptic techniques, including the use of appropriate personal protective equipment, to complete tasks while avoiding contamination from the body or laboratory environment.
2. Use serological pipette to accurately dispense media into culture tube.
3. Inoculate bacteria into fresh liquid media and incubate for growth.
4. Obtain isolated colonies by streaking from a broth bacterial culture onto the surface of an agar plate.
5. Obtain isolated colonies by re-streaking from a previously-grown bacterial colony onto the surface of an agar plate.